Purposeexpression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||58977||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
Currently unavailable outside the U.S.
This material is available to academics and nonprofits only.
Vector backboneT7-VEE-IRES-puro (Addgene plasmid 58970)
Backbone manufacturerDowdy lab
Vector typeMammalian Expression ; Venezuelan equine encephalitis (VEE) virus RNA replicon
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
- Promoter 26S subgenomic promoter
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer pCDH-rev (GCATTCCTTTGGCGAGAG) (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:T7-VEE-GFP was a gift from Steven Dowdy (Addgene plasmid # 58977 ; http://n2t.net/addgene:58977 ; RRID:Addgene_58977)
For your References section:Efficient generation of human iPSCs by a synthetic self-replicative RNA. Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, Chi NC, Fu XD, Yu BD, Dowdy SF. Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 10.1016/j.stem.2013.06.001. 10.1016/j.stem.2013.06.001 PubMed 23910086