Purpose(Empty Backbone) noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||58970||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
Currently unavailable outside the U.S.
This material is available to academics and nonprofits only.
Vector backboneVEE backbone
Backbone manufacturergift from I. Frolov
- Backbone size (bp) 10793
Modifications to backboneadded IRES-puro
Vector typeMammalian Expression ; Venezuelan equine encephalitis (VEE) virus RNA replicon
- Promoter 26S subgenomic promoter
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
- Cloning method Unknown
- 5′ sequencing primer N/A
- 3′ sequencing primer pCDH-rev GCATTCCTTTGGCGAGAG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:T7-VEE-IRES-Puro was a gift from Steven Dowdy (Addgene plasmid # 58970 ; http://n2t.net/addgene:58970 ; RRID:Addgene_58970)
For your References section:Efficient generation of human iPSCs by a synthetic self-replicative RNA. Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, Chi NC, Fu XD, Yu BD, Dowdy SF. Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 10.1016/j.stem.2013.06.001. 10.1016/j.stem.2013.06.001 PubMed 23910086