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A more efficient method to generate integration-free human iPS cells.
Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi M, Takahashi J, Saji H, Yamanaka S
Nat Methods. 2011 May;8(5):409-12
PubMed Article

Plasmids from Article

ID Plasmid Purpose
27076pCXLE-hOCT3/4Non-integrating (episomal) expression of human OCT3/4
27077pCXLE-hOCT3/4-shp53-FIntegration-free (episomal) expression of human OCT3/4 and shRNA against p53
27078pCXLE-hSKIntegration-free (episomal) expression of human SOX2 and KLF4
27079pCXLE-hMLNNon-integrating (episomal) expression of human C-MYC, LIN28 and NANOG
27080pCXLE-hULIntegration-free (episomal) expression of human L-MYC and LIN28
27082pCXLE-EGFPIntegration-free (episomal) expression of EGFP

Antibodies from Article