BIOFAB plasmid set
Depositing Lab: Drew Endy
This is a collection of precisely quantitated bacterial transcription and translation initiation elements. The kit contains combinations of specific constitutive bacterial promoters that vary in strength, followed by a specific Bi-Cistronic Design element that also varies in effectiveness. These different, unique combinations can be used to reliably drive expression of a gene of interest in E. coli in a controlled, reliable manner.
This kit consists of one 96-well plate, and will be shipped as bacterial glycerol stocks on dry ice. Samples should be frozen at -80°C immediately upon arrival.
Please note that plasmids in wells A1-C10 (pFAB3905-pFAB3893) are shipped as glycerol stocks in strain BW25113. Plasmids in wells C11-E1 (pFAB801-pFAB1935) are shipped as glycerol stocks in strain DH10B.