PurposeBIOFAB RFP reporter plasmid for measuring promoter 14 + BCD21 efficiency.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||47828||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namepromoter 14 and BCD21
Alt nameP14, apFAB154 and BCD21, apFAB699
Insert Size (bp)124
- Promoter see insert
- Cloning method Unknown
- 5′ sequencing primer Neo-R
- 3′ sequencing primer p15A-R (Common Sequencing Primers)
RFP fluorescence (Arbitrary Units, AU) is used to measure the efficiency of the promoter/BCD combination. Promoter/BCD combinations ranged from a low score of 6.14 to a high score of 634.54. The score for this combination was: 51.15
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFAB3997 was a gift from Drew Endy (Addgene plasmid # 47828 ; http://n2t.net/addgene:47828 ; RRID:Addgene_47828)
For your References section:Precise and reliable gene expression via standard transcription and translation initiation elements. Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10. 10.1038/nmeth.2404 PubMed 23474465