This website uses cookies to ensure you get the best experience. By continuing the use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

AICSDP-9:DSP-mEGFP
(Plasmid #87424)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 87424 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DSP Homology Arms with linker-mEGFP
  • Alt name
    DSP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2738
  • Mutation
    homology arms contain point mutations to disrupt crRNA binding sites used and point mutations specific to WTC genome
  • Entrez Gene
    DSP (a.k.a. DCWHKTA, DP)
  • Tag / Fusion Protein
    • linker-mEGFP (C terminal on insert)

Cloning Information

Resource Information

Depositor Comments

This plasmid has been used with locus-specific CRISPR/Cas9 to add an mEGFP tag to the C-terminus of human DSP in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: HDPPVAT. Please see http://www.allencell.org/ for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AICSDP-9:DSP-mEGFP was a gift from The Allen Institute for Cell Science (Addgene plasmid # 87424)
  • For your References section:

    Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization. Roberts B, Haupt A, Tucker A, Grancharova T, Arakaki J, Fuqua M, Nelson A, Hookway C, Ludmann S, Mueller I, Yang R, Horwitz A, Rafelski S, Gunawardane R. bioRxiv 2017 10.1101/123042