Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization.
Roberts B, Haupt A, Tucker A, Grancharova T, Arakaki J, Fuqua MA, Nelson A, Hookway C, Ludmann SA, Mueller IA, Yang R, Horwitz R, Rafelski SM, Gunawardane RN
Mol Biol Cell. 2017 Oct 15;28(21):2854-2874. doi: 10.1091/mbc.E17-03-0209. Epub 2017 Aug 16. PubMed Article
Roberts B, Haupt A, Tucker A, Grancharova T, Arakaki J, Fuqua MA, Nelson A, Hookway C, Ludmann SA, Mueller IA, Yang R, Horwitz R, Rafelski SM, Gunawardane RN
Mol Biol Cell. 2017 Oct 15;28(21):2854-2874. doi: 10.1091/mbc.E17-03-0209. Epub 2017 Aug 16. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 87420 | AICSDP-1:PXN-EGFP | Homology arms and linker-EGFP sequence for C-terminus tagging of human PXN |
| 87421 | AICSDP-4:TUBA1B-mEGFP | Homology arms and linker-mEGFP sequence for N-terminus (second exon) tagging of human TUBA1B |
| 87422 | AICSDP-10:LMNB1-mEGFP | Homology arms and linker-mEGFP sequence for N-terminus tagging of human LMNB1 |
| 87423 | AICSDP-8:TOMM20-mEGFP | Homology arms and linker-mEGFP sequence for C-terminus tagging of human TOMM20 |
| 87424 | AICSDP-9:DSP-mEGFP | Homology arms and linker-mEGFP sequence for C-terminus tagging of human DSP |
| 87425 | AICSDP-15:ACTB-mEGFP | Homology arms and linker-mEGFP sequence for N-terminus tagging of human ACTB |
| 87426 | AICSDP-7:SEC61B-mEGFP | Homology arms and linker-mEGFP sequence for N-terminus tagging of human SEC61B |
| 87427 | AICSDP-13:FBL-mEGFP | Homology arms and linker-mEGFP sequence for C-terminus tagging of human FBL |
| 87428 | AICSDP-24:MYH10-mEGFP | Homology arms and linker-mEGFP sequence for N-terminus tagging of human MYH10 |
| 87429 | AICSDP-23:TJP1-mEGFP | Homology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1 |