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(Plasmid #87420)


Item Catalog # Description Quantity Price (USD)
Plasmid 87420 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    New England BioLabs
  • Vector type
    Mammalian Expression, CRISPR ; Donor Template

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    PXN Homology Arms with linker-EGFP
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    homology arms contain point mutations to disrupt crRNA binding sites used
  • Entrez Gene
  • Tag / Fusion Protein
    • linker-EGFP (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Depositor Comments

This plasmid has been used with locus-specific CRISPR/Cas9 to add an EGFP tag to the C-terminus of human PXN in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: GTSGGS. Please see for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AICSDP-1:PXN-EGFP was a gift from The Allen Institute for Cell Science (Addgene plasmid # 87420)
  • For your References section:

    Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization. Roberts B, Haupt A, Tucker A, Grancharova T, Arakaki J, Fuqua M, Nelson A, Hookway C, Ludmann S, Mueller I, Yang R, Horwitz A, Rafelski S, Gunawardane R. bioRxiv 2017 10.1101/123042