|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||87425||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, CRISPR ; Donor Template
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameACTB Homology Arms with mEGFP-linker
SpeciesH. sapiens (human)
Insert Size (bp)2729
Mutationhomology arms contain point mutations to disrupt crRNA binding sites used and point mutations specific to WTC genome
Entrez GeneACTB (a.k.a. BRWS1, PS1TP5BP1)
/ Fusion Protein
- mEGFP-linker (N terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13-Rev (Common Sequencing Primers)
Terms and Licenses
This plasmid has been used with locus-specific CRISPR/Cas9 to add an mEGFP tag to the N-terminus of human ACTB in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: AGSGT, Please see http://www.allencell.org/ for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AICSDP-15:ACTB-mEGFP was a gift from The Allen Institute for Cell Science (Addgene plasmid # 87425)
For your References section:Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization. Roberts B, Haupt A, Tucker A, Grancharova T, Arakaki J, Fuqua M, Nelson A, Hookway C, Ludmann S, Mueller I, Yang R, Horwitz A, Rafelski S, Gunawardane R. bioRxiv 2017 10.1101/123042
Generated by Addgene from full sequence.