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AICSDP-4:TUBA1B-mEGFP
(Plasmid #87421)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 87421 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC19
  • Backbone manufacturer
    New England BioLabs
  • Vector type
    Mammalian Expression, CRISPR ; Donor Template

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TUBA1B Homology Arms with mEGFP-linker
  • Alt name
    TUBA1B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2750
  • Mutation
    homology arms contain point mutations to disrupt crRNA binding sites used
  • Entrez Gene
    TUBA1B (a.k.a. K-ALPHA-1)
  • Tag / Fusion Protein
    • mEGFP-linker (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Depositor Comments

This plasmid has been used with locus-specific CRISPR/Cas9 to add an mEGFP tag to the second exon of human TUBA1B in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: GGSGGS. Please see http://www.allencell.org/ for more information. For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AICSDP-4:TUBA1B-mEGFP was a gift from The Allen Institute for Cell Science (Addgene plasmid # 87421)
  • For your References section:

    Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization. Roberts B, Haupt A, Tucker A, Grancharova T, Arakaki J, Fuqua MA, Nelson A, Hookway C, Ludmann SA, Mueller IA, Yang R, Horwitz R, Rafelski SM, Gunawardane RN. Mol Biol Cell. 2017 Oct 15;28(21):2854-2874. doi: 10.1091/mbc.E17-03-0209. Epub 2017 Aug 16. 10.1091/mbc.E17-03-0209 PubMed 28814507