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(Plasmid #114411)


Item Catalog # Description Quantity Price (USD)
Plasmid 114411 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Mammalian Expression, CRISPR ; Donor Template

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    TNNI1 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    TNNI1 (a.k.a. SSTNI, TNN1)
  • Tag / Fusion Protein
    • linker-mEGFP (C terminal on insert)

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid has been used with locus-specific CRISPR/Cas9 in a two-step editing process to add a mEGFP tag to the C-terminus of human TNNI1 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. After initial HDR, stable expression of hPGK-driven mCherry will indicate cells with integrated donor sequence. This selection cassette is designed to be removed with subsequent CRISPR/Cas9-mediated excision as previously described ( A linker of 3-21 amino acids will be produced after excision. To enrich for edited cells, see our protocol for fluorsecence-assisted cell sorting and subcloning of hiPSCs ( Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (

For more information on the entire plasmid collection, please see

Please visit for the bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AICSDP-34: TNNI1-mEGFP was a gift from Allen Institute for Cell Science (Addgene plasmid # 114411 ; ; RRID:Addgene_114411)
  • For your References section:

    Fluorescent Gene Tagging of Transcriptionally Silent Genes in hiPSCs. Roberts B, Hendershott MC, Arakaki J, Gerbin KA, Malik H, Nelson A, Gehring J, Hookway C, Ludmann SA, Yang R, Haupt A, Grancharova T, Valencia V, Fuqua MA, Tucker A, Rafelski SM, Gunawardane RN. Stem Cell Reports. 2019 May 14;12(5):1145-1158. doi: 10.1016/j.stemcr.2019.03.001. Epub 2019 Apr 4. 10.1016/j.stemcr.2019.03.001 PubMed 30956114