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pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE
(Plasmid #162381)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 162381 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    AAV-CAG-FLEX
  • Backbone manufacturer
    Scott Sternson
  • Vector type
    Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    jGCaMP8m
  • Alt name
    variant 686
  • Species
    R. norvegicus (rat), G. gallus (chicken); A. victoria (jellyfish)
  • Insert Size (bp)
    1269
  • Mutation
    A25G F286Y
  • Promoter CAG
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE was a gift from GENIE Project (Addgene plasmid # 162381 ; http://n2t.net/addgene:162381 ; RRID:Addgene_162381)
  • For your References section:

    jGCaMP8 Fast Genetically Encoded Calcium Indicators. Zhang , Yan; Rózsa, Márton; Bushey, Daniel; Zheng, Jihong; Reep, Daniel; Broussard, Gerard Joey; Tsang, Arthur; Tsegaye, Getahun; Patel, Ronak; Narayan, Sujatha; Li, Jing Xuan; Zhang, Rongwei; Ahrens, Misha B.; Turner, Glenn C.; Wang, Samuel S.-H.; Svoboda, Karel; Korff, Wyatt; Schreiter, Eric R.; Hasseman, Jeremy P.; Kolb, Ilya; Looger, Loren L. Janelia Research Campus. Online resource. 2020 10.25378/janelia.13148243.v1