pAAV-CAG-eYFP
(Plasmid
#104055)
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PurposeAn AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 104055 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAAV
- Backbone size w/o insert (bp) 5176
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEYFP
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Insert Size (bp)720
- Promoter CAG
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
Resource Information
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Terms and Licenses
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Industry Terms
- Not Available to Industry
Depositor Comments
Please visit https://www.biorxiv.org/content/early/2018/01/19/246405 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-CAG-eYFP was a gift from Viviana Gradinaru (Addgene plasmid # 104055 ; http://n2t.net/addgene:104055 ; RRID:Addgene_104055) -
For your References section:
Systemic AAV vectors for widespread and targeted gene delivery in rodents. Challis RC, Ravindra Kumar S, Chan KY, Challis C, Beadle K, Jang MJ, Kim HM, Rajendran PS, Tompkins JD, Shivkumar K, Deverman BE, Gradinaru V. Nat Protoc. 2019 Feb;14(2):379-414. doi: 10.1038/s41596-018-0097-3. 10.1038/s41596-018-0097-3 PubMed 30626963