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viral-icon-01.png Viral Service: PHP.eB AAV

As part of our Viral Service, Addgene is producing ready-to-use viral preparations in the PHP.eB serotype from the Viviana Gradinaru laboratory. These viral vector preparations were produced with the pUCmini-iCAP-PHP.eB plasmid (Addgene #103005).

The PHP.eB serotype exhibits efficient transduction of the central nervous system via systemic delivery in adult animals. In vivo studies showed that, compared to PHP.B and AAV9, intravenous injection of PHP.eB AAV led to an increase in both the number of transduced cells and the expression level per cell. In vivo, PHP.eB transduced the majority of neurons in the cortex and striatum, and over 75% of cerebellar Purkinje cells.

As part of our standard viral production, these viral vectors undergo quality control, including AAV titering by SYBR green qPCR, confirmation of the transfer plasmid with PCR, purity assessment by SYPRO Red staining, in vitro and (when possible) in vivo testing, and full sequencing of the final viral vector preparation.

Note on PHPeB tropism and specific mouse lines: Enhanced CNS tropism exhibited by the PHPeB serotype has been reported to occur through the cellular receptor LY6A (DOI 538421). For information on whether the PHPeB serotype will work in your mouse line, see Supplementary Table 3.

Browse Available PHP.eB AAV

ID Name Promoter Activity Category PI
28306 pAAV-FLEX-tdTomato CAG tdTomato, Cre-dependent Control Boyden
59462 pAAV-CAG-tdTomato CAG tdTomato Control Boyden
28304 pAAV-FLEX-GFP CAG GFP, Cre-dependent Control Boyden
37825 pAAV-CAG-GFP CAG GFP Control Boyden
104061 CAG-NLS-GFP CAG NLS-GFP Control Gradinaru
44361 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Syn1 Activator, Cre-dependent DREADD Roth
44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Syn1 Inhibitor, Cre-dependent DREADD Roth
104491 pGP-AAV-syn-FLEX-jGCaMP7s-WPRE Syn Calcium sensor, Cre-dependent GCaMP Kim
104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE Syn Calcium sensor, Cre-dependent GCaMP Kim
105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn Cre-EGFP expression Recombinases Wilson

Citation Information

When using the PHP.eB serotype in future publications, please acknowledge Viviana Gradinaru and Benjamin Deverman and cite Chan et al., Nat Neurosci, 20(8):1172-1179. Pubmed.

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Please let us know what you would like to see available in viral format. Please note this does not guarantee viral service, but lets us know what viruses would be helpful to the scientific community.

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