pUCmini-iCAP-AAV9-X1.1
(Plasmid #196836)

• Purpose: non-standard AAV2 rep-AAV9-X1.1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system
• Depositing Lab: Viviana Gradinaru
• Publication: Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi: 10.1038/s41467-023-38582-7.

Backbone

• Vector backbone: pUC57-mini
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Synthetic construct isolate AAV9-X1.1 VP1 gene
• Alt name: AAV9-X1.1 cap
• Species: Synthetic
• Promoter: p41

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • pUC57mini-iCap-X1.1.gbk
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This work supported by NIH BRAIN Initiative grant UF1MH128336. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.