kiCAP-AAV-BI30
(Plasmid #183749)

• Purpose: AAV-BI30 Rep-Cap plasmid for production of AAV-BI30, a capsid with tropism for CNS endothelial cells.
• Depositing Lab: Ben Deverman
• Publication: Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-400. doi: 10.1038/s44161-022-00046-4. Epub 2022 Apr 13.

Backbone

• Vector backbone: pUC57-Kan
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 9398
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AAV9 modified with 7mer insertion between amino acids 588 and 589 of VP1
• Alt name: BI30
• Species: Synthetic
• Insert Size (bp): 2232
• Mutation: K449R, and NNSTRGG inserted between 588 and 589 of AAV9 VP1
• Promoter: p41

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsiWI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: NA
• 3′ sequencing primer: NA

Resource Information

• Supplemental Documents:
  • iCAP-kan-BI30.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry