CN3863: rAAV-core4-eHGT_888m-minBglobin-SYFP2-WPRE3-BGHpA
(Plasmid
#199773)
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Purposeenhancer AAV for expression of SYFP2 in midbrain dopamine neurons
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 199773 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonerAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSYFP2
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SpeciesSynthetic
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Insert Size (bp)720
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MutationwtGFP plus mutations F46L F64L S65G S72A M153T V163A S175G T203Y A206K
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GenBank IDDQ092361
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bySYFP2 was PCR amplified from Addgene plasmid #22905
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This work supported by NIH BRAIN Initiative grant UF1MH128339
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CN3863: rAAV-core4-eHGT_888m-minBglobin-SYFP2-WPRE3-BGHpA was a gift from The Allen Institute for Brain Science & Jonathan Ting (Addgene plasmid # 199773 ; http://n2t.net/addgene:199773 ; RRID:Addgene_199773)
