AAV-hSyn-Soma-jGCaMP8f
(Plasmid
#169258)
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PurposeAAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8f.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 169258 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGP-AAV-syn-jGCaMP8f-WPRE
- Total vector size (bp) 6182
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSoma-jGCaMP8f
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SpeciesM. musculus (mouse)
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Insert Size (bp)1621
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Tag
/ Fusion Protein
- 6xHis-tag
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bypGP-AAV-syn-jGCaMP8f-WPRE was a gift from GENIE Project. pAAV-Syn-SomaGCaMP7 was a gift from Edward Boyden (#158759)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-hSyn-Soma-jGCaMP8f was a gift from Marianne Fyhn (Addgene plasmid # 169258 ; http://n2t.net/addgene:169258 ; RRID:Addgene_169258) -
For your References section:
An updated suite of viral vectors for in vivo calcium imaging using intracerebral and retro-orbital injections in male mice. Grodem S, Nymoen I, Vatne GH, Rogge FS, Bjornsdottir V, Lensjo KK, Fyhn M. Nat Commun. 2023 Feb 4;14(1):608. doi: 10.1038/s41467-023-36324-3. 10.1038/s41467-023-36324-3 PubMed 36739289
Map uploaded by the depositor.
