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An updated suite of viral vectors for in vivo calcium imaging using intracerebral and retro-orbital injections in male mice.
Grodem S, Nymoen I, Vatne GH, Rogge FS, Bjornsdottir V, Lensjo KK, Fyhn M
Nat Commun. 2023 Feb 4;14(1):608. doi: 10.1038/s41467-023-36324-3. PubMed Article

Plasmids from Article

ID Plasmid Purpose
167572AAV-hSyn-Ribo-jGCaMP8sPlease see Addgene item #169247 for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s.
167573AAV-hSyn-Ribo-jGCaMP8fPlease see Addgene item #169249 (coming soon) for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f.
167574AAV-hSyn-Ribo-jGCaMP8mPlease see Addgene item #169248 for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m.
169247AAV-hSyn-RiboL1-jGCaMP8sAAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.
169248AAV-hSyn-RiboL1-jGCaMP8mAAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8m protein.
169249AAV-hSyn-RiboL1-jGCaMP8fAAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8f protein.
169256AAV-hSyn-Soma-jGCaMP8sAAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8s.
169257AAV-hSyn-Soma-jGCaMP8mAAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8m.
169258AAV-hSyn-Soma-jGCaMP8fAAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8f.
169259AAV-hSyn-jRex-GECOAAV transfer plasmid for neuronal expression of jRex-GECO.
172714AAV-hSyn-Axon-jRex-GECOAAV transfer plasmid for neuronal expression of axon-targeted jRex-GECO.
172719AAV-hSyn-Axon-jGCaMP8mAAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8m.
172720AAV-hSyn-Axon-jGCaMP8sAAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8s.
192611pAAV-CAG-RiboL1-jGCaMP8sAAV transfer plasmid for CAG mediated expression of soma-targeted (RiboL1) jGCaMP8s.
192612pAAV-CAG-RiboL1-jGCaMP8fAAV transfer plasmid for CAG mediated expression of soma-targeted (RiboL1) jGCaMP8f.
192613pAAV-CAG-RiboL1-jGCaMP8mAAV transfer plasmid for CAG mediated expression of soma-targeted (RiboL1) jGCaMP8m.
192615AAV-hSyn-Axon-jGCaMP8fAAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8f.
192616pAAV-hSyn-FLEX-RiboL1-jGCaMP8sAAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8s.
192617pAAV-hSyn-FLEX-RiboL1-jGCaMP8mAAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8m.
192618pAAV-hSyn-FLEX-RiboL1-jGCaMP8fAAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8f.
192619pAAV-hSyn-RiboL1-jGCaMP7cAAV transfer plasmid for neuronal expression of soma-targeted (RiboL1) jGCaMP7c.

Antibodies from Article