Addgene - pACAGW-ChR2-Venus-AAV Plasmid Data

Plasmid 20071: pACAGW-ChR2-Venus-AAV

Gene/insert name:
Channelrhodopsin-2
Alt name:
ChR2
Alt name:
Channelrhodopsin
Insert size:
3088
Species:
Chlamydomonas reinhardtii
Entrez Gene:
COP4 (CHLREDRAFT_182032)
Fusion protein or tag:
Venus
Terminal:
C terminal on insert
Vector backbone:
N/A
(Search Vector Database)
Vector type:
Mammalian Expression ; AAV
Backbone size w/o insert (bp):
3824
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Grow on Stbl2 cells
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
Georg Nagel (MPI Biophysics)
Sequence: View sequences (3)
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Karel Svoboda
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The subcellular organization of neocortical excitatory connections. Leopoldo et al (Nature. 2009 Jan 18. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20071" in your Materials and Methods section.