pACAGW-ChR2-Venus-AAV
(Plasmid #20071)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    N/A
  • Backbone size w/o insert (bp) 3824
  • Vector type
    Mammalian Expression ; AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Grow on Stbl2 cells
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    Channelrhodopsin-2
  • Alt name
    ChR2
  • Alt name
    Channelrhodopsin
  • Species
    Chlamydomonas reinhardtii
  • Insert Size (bp)
    3088
  • Entrez Gene
    COP4 (a.k.a. CHLREDRAFT_182032)
  • Tag / Fusion Protein
    • Venus (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACAGW-ChR2-Venus-AAV was a gift from Karel Svoboda (Addgene plasmid # 20071)
  • For your References section:

    The subcellular organization of neocortical excitatory connections. Leopoldo Petreanu, Tianyi Mao, Scott M. Sternson & Karel Svoboda. Nature. 2009 Jan 18. 10.1038/nature07709 PubMed 19151697