Plasmid 20938: pcDNA3.1/hChR2(H134R)-mCherry
  • Mammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

  • channelrhodopsin-2

  • ChR2

  • 1647

  • C. reinhardtii

  • H134R mutation in ChR2

  • pcDNA3.1
    (Search Vector Database)

  • Mammalian Expression

  • 5545

  • HindIII

  • Unknown

  • XbaI

  • Unknown

  • n/a List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (4)
  • Karl Deisseroth

    Clontech Limited Use Label License
    Ancillary Agreement for Plasmids Containing FP Materials


Please note that the depositor's sequence is missing the H134R mutation, however Addgene's quality control sequence confirmed this mutation.

The H134R mutation present in the ChR2 sequence is a gain-of-function mutant that produces larger stationary photocurrents in comparison to the wild-type ChR2 sequence

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Multimodal fast optical interrogation of neural circuitry. Zhang et al (Nature. 2007 Apr 5. 446(7136):633-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20938" in your Materials and Methods section.