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Plasmid 20938: pcDNA3.1/hChR2(H134R)-mCherry
  • Mammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

  • channelrhodopsin-2

  • ChR2

  • 1647

  • C. reinhardtii

  • H134R mutation in ChR2

  • pcDNA3.1
    (Search Vector Database)

  • Mammalian Expression

  • 5545

  • HindIII

  • Unknown

  • XbaI

  • Unknown

  • n/a List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (4)
  • Karl Deisseroth

  • UBMTA
    Clontech Limited Use Label License
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

Please note that the depositor's sequence is missing the H134R mutation, however Addgene's quality control sequence confirmed this mutation.

The H134R mutation present in the ChR2 sequence is a gain-of-function mutant that produces larger stationary photocurrents in comparison to the wild-type ChR2 sequence

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Multimodal fast optical interrogation of neural circuitry. Zhang et al (Nature. 2007 Apr 5. 446(7136):633-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20938" in your Materials and Methods section.