PurposeMammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20938||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5545
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)1647
MutationH134R mutation in ChR2
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Please note that the depositor's sequence is missing the H134R mutation, however Addgene's quality control sequence confirmed this mutation.
The H134R mutation present in the ChR2 sequence is a gain-of-function mutant that produces larger stationary photocurrents in comparison to the wild-type ChR2 sequence
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3.1/hChR2(H134R)-mCherry was a gift from Karl Deisseroth (Addgene plasmid # 20938)
For your References section:Multimodal fast optical interrogation of neural circuitry. Zhang F, Wang LP, Brauner M, Liewald JF, Kay K, Watzke N, Wood PG, Bamberg E, Nagel G, Gottschalk A, Deisseroth K. Nature. 2007 Apr 5. 446(7136):633-9. 10.1038/nature05744 PubMed 17410168
Generated by Addgene from full sequence supplied by depositor.