pcDNA3.1/hChR2(H134R)-mCherry
(Plasmid #20938)

Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    channelrhodopsin-2
  • Alt name
    ChR2
  • Species
    C. reinhardtii
  • Insert Size (bp)
    1647
  • Mutation
    H134R mutation in ChR2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please note that the depositor's sequence is missing the H134R mutation, however Addgene's quality control sequence confirmed this mutation.

The H134R mutation present in the ChR2 sequence is a gain-of-function mutant that produces larger stationary photocurrents in comparison to the wild-type ChR2 sequence

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1/hChR2(H134R)-mCherry was a gift from Karl Deisseroth (Addgene plasmid # 20938)
  • For your References section:

    Multimodal fast optical interrogation of neural circuitry. Zhang F, Wang LP, Brauner M, Liewald JF, Kay K, Watzke N, Wood PG, Bamberg E, Nagel G, Gottschalk A, Deisseroth K. Nature. 2007 Apr 5. 446(7136):633-9. 10.1038/nature05744 PubMed 17410168