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pAAV-mDlx-ChR2-mCherry-Fishell-3
(Plasmid #83898)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 83898 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ChR2
  • Promoter mDlx

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer chim-int-F (5'-TCTTACTGACATCCACTTTGCC-3')
  • 3′ sequencing primer WPRE-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-mDlx-ChR2-mCherry-Fishell-3 was a gift from Gordon Fishell (Addgene plasmid # 83898)
  • For your References section:

    A viral strategy for targeting and manipulating interneurons across vertebrate species. Dimidschstein J, Chen Q, Tremblay R, Rogers SL, Saldi GA, Guo L, Xu Q, Liu R, Lu C, Chu J, Avery MC, Rashid MS, Baek M, Jacob AL, Smith GB, Wilson DE, Kosche G, Kruglikov I, Rusielewicz T, Kotak VC, Mowery TM, Anderson SA, Callaway EM, Dasen JS, Fitzpatrick D, Fossati V, Long MA, Noggle S, Reynolds JH, Sanes DH, Rudy B, Feng G, Fishell G. Nat Neurosci. 2016 Oct 31. doi: 10.1038/nn.4430. 10.1038/nn.4430 PubMed 27798629