|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||102417||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3873
- Total vector size (bp) 5338
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesD. rerio (zebrafish)
Insert Size (bp)1465
- Promoter Zebrafish E1b minimal promoter
- Cloning method Gibson Cloning
- 5′ sequencing primer ATCCCGTACCGACCGTTATC
- 3′ sequencing primer GGTAATGGTAGCGACCGGC (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byPlasmid #37846 (N. Ahituv) and parts of plasmid obtained directly from the authors listed, via MTA dated 27th August 2012.
Terms and Licenses
- Not Available to Industry
Article Citing this Plasmid
A. Emelyanov and S. Parinov. Mifepristone-inducible LexPR system to drive and control gene expression in transgenic zebrafish. Developmental Biology, 320(1):113– 121, 2008. ISSN 00121606. doi: 10.1016/j.ydbio.2008.04.042.
A. Emelyanov, Y. Gao, N. I. Naqvi, and S. Parinov. Trans-kingdom transposition of the maize Dissociation element. Genetics, 174(3):1095–1104, 2006. ISSN 00166731. doi: 10.1534/genetics.106.061184.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pVC-Ds-E1b:eGFP-Ds was a gift from Tatjana Sauka-Spengler (Addgene plasmid # 102417 ; http://n2t.net/addgene:102417 ; RRID:Addgene_102417)
For your References section:Re-purposing Ac/Ds transgenic system for CRISPR/dCas9 modulation of enhancers and non-coding RNAs in zebrafish. Chong-Morrison V, Simões FC, Senanayake U, Carroll DS, Riley PR, Sauka-Spengler T. bioRxiv 10.1101/450684