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Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs.
Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess SM, Patton JG, Chen W
Genetics. 2015 Apr 8. pii: genetics.115.176917.
PubMed Article

Plasmids from Article

ID Plasmid  
64237pME-Cas9
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64239pGGDestTol2LC-1sgRNA
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64240pGGDestTol2LC-2sgRNA
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64241pGGDestTol2LC-3sgRNA
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64242pGGDestTol2LC-4sgRNA
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64243pGGDestTol2LC-5sgRNA
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64245pU6a:sgRNA#1
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64246pU6a:sgRNA#2
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64247pU6b:sgRNA#3
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64248pU6c:sgRNA#4
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64249pU6d:sgRNA#5
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64250pU6a:sgRNA(tyr)
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