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pOTTC1329 - pAAV EF1a HA-hM4D(Gi)
(Plasmid #89150)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89150 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV EF1a DIO
  • Backbone manufacturer
    Karl Deisseroth
  • Backbone size w/o insert (bp) 5239
  • Modifications to backbone
    lox sites were removed when CDS was added between BamHI and EcoRV
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HA-tagged hM4D(Gi)
  • Alt name
    Gq-coupled inhibitory DREADD
  • Promoter EF1a
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer EF1a F1
  • 3′ sequencing primer WPRE R1
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOTTC1329 - pAAV EF1a HA-hM4D(Gi) was a gift from Brandon Harvey (Addgene plasmid # 89150 ; http://n2t.net/addgene:89150 ; RRID:Addgene_89150)
  • For your References section:

    Chemogenetics revealed: DREADD occupancy and activation via converted clozapine. Gomez JL, Bonaventura J, Lesniak W, Mathews WB, Sysa-Shah P, Rodriguez LA, Ellis RJ, Richie CT, Harvey BK, Dannals RF, Pomper MG, Bonci A, Michaelides M. Science. 2017 Aug 4;357(6350):503-507. doi: 10.1126/science.aan2475. 10.1126/science.aan2475 PubMed 28774929