-
PurposeGi-coupled hM4D-IRES-mCitrine under the control of human synapsin promote
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
---|---|---|---|---|---|---|
Plasmid | 50464 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $65 |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepAAV
- Backbone size w/o insert (bp) 4818
- Total vector size (bp) 7610
-
Vector typeAAV
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert namehM4D(Gi)-IRES-mCitrine
-
SpeciesH. sapiens (human)
-
Insert Size (bp)2792
-
Entrez GeneCHRM4 (a.k.a. HM4, M4R)
- Promoter human Synapsin 1
-
Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Asc I (not destroyed)
- 3′ cloning site Nhe I (not destroyed)
- 5′ sequencing primer TCGTGTCGTGCCTGAGAGCG
- 3′ sequencing primer GCATTAAAGCAGCGTATCCACATAGC (Common Sequencing Primers)
Resource Information
-
Terms and Licenses
-
Articles Citing this Plasmid
Depositor Comments
For more information, see: http://pdspit3.mml.unc.edu/projects/dreadd/wiki/WikiStart
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAAV-hSyn-HA-hM4D(Gi)-IRES-mCitrine was a gift from Bryan Roth (Addgene plasmid # 50464 ; http://n2t.net/addgene:50464 ; RRID:Addgene_50464)