pAAV-CaMKIIa-HA-KORD-IRES-mCitrine
(Plasmid #65418)

• Purpose: AAV packaging vector for CamKIIa driven KOR DREADD expression
• Depositing Lab: Bryan Roth
• Publication: Vardy et al Neuron. 2015 Apr 29. pii: S0896-6273(15)00289-5. doi: 10.1016/j.neuron.2015.03.065.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	65418	Standard format: Plasmid sent in bacteria as agar stab	1	$65	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pAAV2
  (Search Vector Database)
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CaMKIIa-HA-KORD-IRES-mCitrine
• Alt name: CamKIIa KOR DREADD
• Species: H. sapiens (human)
• Entrez Gene: OPRK1 (a.k.a. K-OR-1, KOR, KOR-1, OPRK)
• Promoter: CaMKIIa
• Tags / Fusion Proteins:
  • HA (N terminal on insert)
  • mCitrine (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: Unknown
• 3′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • pAAV-CaMKIIa-HA-KORD-IRES-mCitrine
• Addgene Notes:
  • Addgene diagnostic digest
• Terms and Licenses:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials