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PurposeAAV packaging vector for CamKIIa driven KOR DREADD expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 65418 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAAV2
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCaMKIIa-HA-KORD-IRES-mCitrine
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Alt nameCamKIIa KOR DREADD
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SpeciesH. sapiens (human)
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Entrez GeneOPRK1 (a.k.a. K-OR-1, KOP, KOR, KOR-1, KOR1, OPRK)
- Promoter CaMKIIa
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Tags
/ Fusion Proteins
- HA (N terminal on insert)
- mCitrine (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown
- 3′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Addgene Notes
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Article Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-CaMKIIa-HA-KORD-IRES-mCitrine was a gift from Bryan Roth (Addgene plasmid # 65418 ; http://n2t.net/addgene:65418 ; RRID:Addgene_65418) -
For your References section:
A New DREADD Facilitates the Multiplexed Chemogenetic Interrogation of Behavior. Vardy E, Robinson JE, Li C, Olsen RH, DiBerto JF, Giguere PM, Sassano FM, Huang XP, Zhu H, Urban DJ, White KL, Rittiner JE, Crowley NA, Pleil KE, Mazzone CM, Mosier PD, Song J, Kash TL, Malanga CJ, Krashes MJ, Roth BL. Neuron. 2015 Apr 29. pii: S0896-6273(15)00289-5. doi: 10.1016/j.neuron.2015.03.065. 10.1016/j.neuron.2015.03.065 PubMed 25937170