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AA0250-mCherry-2a-hM4D-nrxn1a rev
(Plasmid #60544)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 60544 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    AAV2
  • Backbone size w/o insert (bp) 5048
  • Total vector size (bp) 7478
  • Vector type
    AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin (50ug/ml)
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    mCherry
  • Insert Size (bp)
    710
  • Promoter CAG

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer pCAG-F
  • 3′ sequencing primer WPRE-R
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    muscarinic receptor 4, axon targeted variant
  • Alt name
    M4R
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1720
  • Entrez Gene
    CHRM4 (a.k.a. HM4, M4R)
  • Promoter CAG
  • Tag / Fusion Protein
    • 2xHA (C terminal on insert)

Cloning Information for Gene/Insert 2

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AA0250-mCherry-2a-hM4D-nrxn1a rev was a gift from Scott Sternson (Addgene plasmid # 60544 ; http://n2t.net/addgene:60544 ; RRID:Addgene_60544)
  • For your References section:

    Chemogenetic synaptic silencing of neural circuits localizes a hypothalamus-->midbrain pathway for feeding behavior. Stachniak TJ, Ghosh A, Sternson SM. Neuron. 2014 May 21;82(4):797-808. doi: 10.1016/j.neuron.2014.04.008. Epub 2014 Apr 24. 10.1016/j.neuron.2014.04.008 PubMed 24768300