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pAAV-PTRE-tight-hM3Dq-mCherry
(Plasmid #66795)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 66795 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 7159
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pTIGHT promoter-hM3Dq-mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    4400
  • Promoter pTIGHT TRE

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (unknown if destroyed)
  • 3′ cloning site NheI (unknown if destroyed)
  • 5′ sequencing primer Tggaaagagaactgtgcctc
  • 3′ sequencing primer Cagttcatgtacggctccaag
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

hM3Dq-mCherry reading frame was obtained from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (Addgene plasmid #44361) and cloned in the sense orientation between the lox sites. Please see the associated publication for additional cloning details.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-PTRE-tight-hM3Dq-mCherry was a gift from William Wisden (Addgene plasmid # 66795 ; http://n2t.net/addgene:66795 ; RRID:Addgene_66795)
  • For your References section:

    Neuronal ensembles sufficient for recovery sleep and the sedative actions of alpha2 adrenergic agonists. Zhang Z, Ferretti V, Guntan I, Moro A, Steinberg EA, Ye Z, Zecharia AY, Yu X, Vyssotski AL, Brickley SG, Yustos R, Pillidge ZE, Harding EC, Wisden W, Franks NP. Nat Neurosci. 2015 Apr;18(4):553-61. doi: 10.1038/nn.3957. Epub 2015 Feb 23. 10.1038/nn.3957 PubMed 25706476