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pAAV-TRE-HTG
(Plasmid #27437)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27437 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV2-MCS
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2887
  • Vector type
    AAV ; adeno-associated viral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    DH5alpha, standard LB buffer,37oC
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    histone-GFP-2A-TVA receptor-2A-rabies glycoprotein
  • Species
    rabies virus
  • Insert Size (bp)
    4175
  • Mutation
    N/A

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-TRE-HTG was a gift from Liqun Luo (Addgene plasmid # 27437 ; http://n2t.net/addgene:27437 ; RRID:Addgene_27437)
  • For your References section:

    Cortical representations of olfactory input by trans-synaptic tracing. Miyamichi K, Amat F, Moussavi F, Wang C, Wickersham I, Wall NR, Taniguchi H, Tasic B, Huang ZJ, He Z, Callaway EM, Horowitz MA, Luo L. Nature. 2010 Dec 22. ():. 10.1038/nature09714 PubMed 21179085