Addgene - pAAV-TRE-HTG Plasmid Data

Plasmid 27437: pAAV-TRE-HTG

Gene/insert name:
histone-GFP-2A-TVA receptor-2A-rabies glycoprotein
Insert size:
4175
Species:
rabies virus
Mutation:
N/A
Vector backbone:
pAAV2-MCS
(Search Vector Database)
Backbone manufacturer:
Stratagene
Vector type:
adeno-associated viral
Backbone size w/o insert (bp):
2887
Cloning site 5':
NotI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
n/a
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
DH5alpha, standard LB buffer,37oC
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
Dr. Ed Callaway at Salk inst.
Sequence: View sequences (4)
Map:
View map
Principal Investigator:
Liqun Luo
Terms and Licenses:
MTA
Tet Systems Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Cortical representations of olfactory input by trans-synaptic tracing. Miyamichi et al (Nature. 2010 Dec 22. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 27437" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

26197: pAAV-EF1a-FLEX-GTB

13775: pCAG-Cre

19712: pMD.RVG.CVS24-N2c

26196: pAAV-FLEX-hGTB

15214: Cerulean