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pAAV-FLEX-hGTB
(Plasmid #26196)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26196 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    Mr. Gene
  • Backbone size w/o insert (bp) 3300
  • Vector type
    AAV, Cre/Lox ; Adeno-associated virus

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Invitrogen One Shot Stbl3 chemically competent cells
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Tet transactivator, enhanced green fluorescent protein, TVA, rabies B19 glycoprotein
  • Alt name
    tTA, itTA, htTA, GFP, eGFP
  • Alt name
    tv-a
  • Alt name
    B19G, RG
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    4100
  • Mutation
    F2A has 2A cleavage site deleted, producing htTA-eGFP fusion. tTA, TVA, and B19G are all mammalian codon optimized

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SbfI (not destroyed)
  • 3′ cloning site BstBI (not destroyed)
  • 5′ sequencing primer LNCX primer
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-FLEX-hGTB was a gift from Edward Callaway (Addgene plasmid # 26196 ; http://n2t.net/addgene:26196 ; RRID:Addgene_26196)
  • For your References section:

    Monosynaptic circuit tracing in vivo through Cre-dependent targeting and complementation of modified rabies virus. Wall NR, Wickersham IR, Cetin A, De La Parra M, Callaway EM. Proc Natl Acad Sci U S A. 2010 Nov 29. ():. 10.1073/pnas.1011756107 PubMed 21115815