Addgene - pAAV-FLEX-hGTB Plasmid Data

Plasmid 26196: pAAV-FLEX-hGTB

Gene/insert name:
Tet transactivator, enhanced green fluorescent protein, TVA, rabies B19 glycoprotein
Alt name:
tTA, itTA, htTA, GFP, eGFP
Alt name:
tv-a
Alt name:
B19G, RG
Insert size:
4100
Species:
H. sapiens (human), M. musculus (mouse)
Mutation:
F2A has 2A cleavage site deleted, producing htTA-eGFP fusion. tTA, TVA, and B19G are all mammalian codon optimized
Vector backbone:
pAAV
(Search Vector Database)
Backbone manufacturer:
Mr. Gene
Vector type:
Cre/Lox ; Adeno-associated virus
Backbone size w/o insert (bp):
3300
Cloning site 5':
SbfI
Site destroyed during cloning:
No
Cloning site 3':
BstBI
Site destroyed during cloning:
No
5' sequencing primer:
LNCX primer List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Invitrogen One Shot Stbl3 chemically competent cells
High or low copy:
Unknown
Sequence: View sequences (4)
Map:
View map
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Edward Callaway
Terms and Licenses:
MTA
Tet Systems Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Monosynaptic circuit tracing in vivo through Cre-dependent targeting and complementation of modified rabies virus. Wall et al (Proc Natl Acad Sci U S A. 2010 Nov 29. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26196" in your Materials and Methods section.