pAAV-FLEX-hGTB
(Plasmid #26196)

• Depositing Lab: Edward Callaway
• Publication: Wall et al Proc Natl Acad Sci U S A. 2010 Nov 29. ():.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	26196	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Discontinued

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pAAV
  (Search Vector Database)
• Backbone manufacturer: Mr. Gene
• Backbone size w/o insert (bp): 3300
• Vector type: AAV, Cre/Lox ; Adeno-associated virus

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Invitrogen One Shot Stbl3 chemically competent cells
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Tet transactivator, enhanced green fluorescent protein, TVA, rabies B19 glycoprotein
• Alt name: tTA, itTA, htTA, GFP, eGFP
• Alt name: tv-a
• Alt name: B19G, RG
• Species: H. sapiens (human), M. musculus (mouse)
• Insert Size (bp): 4100
• Mutation: F2A has 2A cleavage site deleted, producing htTA-eGFP fusion. tTA, TVA, and B19G are all mammalian codon optimized

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SbfI (not destroyed)
• 3′ cloning site: BstBI (not destroyed)
• 5′ sequencing primer: LNCX primer

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Terms and Licenses:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
  • genOway Notice of RIghts
• Article Citing this Plasmid:
  • 1 Reference