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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 26196 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
Currently unavailable outside the U.S.
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAAV
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Backbone manufacturerMr. Gene
- Backbone size w/o insert (bp) 3300
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Vector typeAAV, Cre/Lox ; Adeno-associated virus
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsInvitrogen One Shot Stbl3 chemically competent cells
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTet transactivator, enhanced green fluorescent protein, TVA, rabies B19 glycoprotein
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Alt nametTA, itTA, htTA, GFP, eGFP
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Alt nametv-a
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Alt nameB19G, RG
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SpeciesH. sapiens (human), M. musculus (mouse)
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Insert Size (bp)4100
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MutationF2A has 2A cleavage site deleted, producing htTA-eGFP fusion. tTA, TVA, and B19G are all mammalian codon optimized
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SbfI (not destroyed)
- 3′ cloning site BstBI (not destroyed)
- 5′ sequencing primer LNCX primer (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-FLEX-hGTB was a gift from Edward Callaway (Addgene plasmid # 26196 ; http://n2t.net/addgene:26196 ; RRID:Addgene_26196) -
For your References section:
Monosynaptic circuit tracing in vivo through Cre-dependent targeting and complementation of modified rabies virus. Wall NR, Wickersham IR, Cetin A, De La Parra M, Callaway EM. Proc Natl Acad Sci U S A. 2010 Nov 29. ():. 10.1073/pnas.1011756107 PubMed 21115815