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pAAV-EF1a-FLEX-GTB
(Plasmid #26197)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26197 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV-double floxed-EYFP-WPRE-pA
  • Backbone manufacturer
    Karl Deisseroth Lab
  • Backbone size w/o insert (bp) 5300
  • Vector type
    AAV, Cre/Lox ; Adeno-associated virus

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Invitrogen One Shot Stbl3 chemically competent cells
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Enhanced green fluorescent protein, TVA, rabies B19 glycoprotein
  • Alt name
    B19G, RG
  • Alt name
    tv-a
  • Alt name
    eGFP
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3250
  • Mutation
    TVA, rabies glycoprotein are mammalian codon optimized

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EF1a_fwd_primer
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-EF1a-FLEX-GTB was a gift from Edward Callaway (Addgene plasmid # 26197 ; http://n2t.net/addgene:26197 ; RRID:Addgene_26197)
  • For your References section:

    Genetic dissection of an amygdala microcircuit that gates conditioned fear. Haubensak W, Kunwar PS, Cai H, Ciocchi S, Wall NR, Ponnusamy R, Biag J, Dong HW, Deisseroth K, Callaway EM, Fanselow MS, Luthi A, Anderson DJ. Nature. 2010 Nov 11. 468(7321):270-6. 10.1038/nature09553 PubMed 21068836