|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32634||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5015
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameB19N, B19P, B19M and B19L
Insert Size (bp)10000
MutationIn frame insertion of Ser after aa10 in M (matrix protein).
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site NA (unknown if destroyed)
- 3′ cloning site NA (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
To construct the rabies virus genomic cDNA, several pieces of rabies genomic cDNA were ligated with unique restriction enzyme sites, AgeI, MluI, BlpI, BstZ17I, and BstBI. Addition of HamRz and HdvRz sequences were performed by PCR using synthesized primers designed to attach these ribozyme sequences.
The pSADdeltaG-F3 has 3 unique restriction enzyme sites Sbf1, Srf1 and NheI for cloning the first gene and 3 unique restriction enzyme sites PacI, AscI and SacII for cloning the second gene between B19M and B19L.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSADdeltaG-F3 was a gift from Edward Callaway (Addgene plasmid # 32634 ; http://n2t.net/addgene:32634 ; RRID:Addgene_32634)
For your References section:New rabies virus variants for monitoring and manipulating activity and gene expression in defined neural circuits. Osakada F, Mori T, Cetin AH, Marshel JH, Virgen B, Callaway EM. Neuron. 2011 Aug 25;71(4):617-31. 10.1016/j.neuron.2011.07.005 PubMed 21867879