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pSADdeltaG-F3
(Plasmid #32634)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 32634 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1/Zeo
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5015
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    B19N, B19P, B19M and B19L
  • Species
    Rabies virus
  • Insert Size (bp)
    10000
  • Mutation
    In frame insertion of Ser after aa10 in M (matrix protein).
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

To construct the rabies virus genomic cDNA, several pieces of rabies genomic cDNA were ligated with unique restriction enzyme sites, AgeI, MluI, BlpI, BstZ17I, and BstBI. Addition of HamRz and HdvRz sequences were performed by PCR using synthesized primers designed to attach these ribozyme sequences.
The pSADdeltaG-F3 has 3 unique restriction enzyme sites Sbf1, Srf1 and NheI for cloning the first gene and 3 unique restriction enzyme sites PacI, AscI and SacII for cloning the second gene between B19M and B19L.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSADdeltaG-F3 was a gift from Edward Callaway (Addgene plasmid # 32634 ; http://n2t.net/addgene:32634 ; RRID:Addgene_32634)
  • For your References section:

    New rabies virus variants for monitoring and manipulating activity and gene expression in defined neural circuits. Osakada F, Mori T, Cetin AH, Marshel JH, Virgen B, Callaway EM. Neuron. 2011 Aug 25;71(4):617-31. 10.1016/j.neuron.2011.07.005 PubMed 21867879