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Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2.
Huo J, Le Bas A, Ruza RR, Duyvesteyn HME, Mikolajek H, Malinauskas T, Tan TK, Rijal P, Dumoux M, Ward PN, Ren J, Zhou D, Harrison PJ, Weckener M, Clare DK, Vogirala VK, Radecke J, Moynie L, Zhao Y, Gilbert-Jaramillo J, Knight ML, Tree JA, Buttigieg KR, Coombes N, Elmore MJ, Carroll MW, Carrique L, Shah PNM, James W, Townsend AR, Stuart DI, Owens RJ, Naismith JH
Nat Struct Mol Biol. 2020 Jul 13. pii: 10.1038/s41594-020-0469-6. doi: 10.1038/s41594-020-0469-6.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
155363pOPINO_NbH11-D4Expresses nanobody H11-D4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2
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155364pOPINO_NbH11-H4Expresses nanobody H11-H4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2
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160313pABVec-NbH11-H4_huIgG1Expresses nanobody H11-H4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2 as a huIgG1 Fc fusion
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160314pABVec-NbH11-D4_huIgG1Expresses Expresses nanobody H11-D4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2 as a huIgG1 Fc fusion
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Antibodies from Article