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Development of CRISPR as an Antiviral Strategy to Combat SARS-CoV-2 and Influenza.
Abbott TR, Dhamdhere G, Liu Y, Lin X, Goudy L, Zeng L, Chemparathy A, Chmura S, Heaton NS, Debs R, Pande T, Endy D, La Russa MF, Lewis DB, Qi LS
Cell. 2020 May 14;181(4):865-876.e12. doi: 10.1016/j.cell.2020.04.020. Epub 2020 Apr 29.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
155303pSLQ5079_pHR_PGK_sfGFP_CoV-F1SARS-CoV-2 fluorescent reporter 1
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155304pSLQ5080_pHR_PGK_sfGFP_CoV-F2SARS-CoV-2 fluorescent reporter 2
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155305pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAGLentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.
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155306pSLQ5429_pUC_hU6-crScaffold_EF1a-BFPRfx Cas13d guide cloning pUC19 vector with constitutively expressed tagBFP
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155307pSLQ5465_pHR_hU6-crScaffold_EF1a-PuroR-T2A-BFPRfx Cas13d guide cloning lentiviral vector with constitutively expressed puromycin resistance 2A-tagged with tagBFP
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