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CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity.
Chen JS, Ma E, Harrington LB, Da Costa M, Tian X, Palefsky JM, Doudna JA
Science. 2018 Apr 27;360(6387):436-439. doi: 10.1126/science.aar6245. Epub 2018 Feb 15.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
113430pMBP-AsCas12aExpresses 10xHis-MBP fused to AsCas12a
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113431pMBP-LbCas12aExpresses 10xHis-MBP fused to LbCas12a
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113432pMBP-FnCas12aExpresses 10xHis-MBP fused to FnCas12a
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113433pMBP-AaCas12bExpresses Expresses 10xHis-MBP fused to AaCas12b
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113517pMBP-LbCas12a D832AExpresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nuclease
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113518pMBP-LbCas12a H759AExpresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processing
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