pTAL5-BB
(Plasmid #36033)

• Purpose: (Empty Backbone)
• Depositing Lab: Tom Ellis
• Publication: Blount et al PLoS One. 2012;7(3):e33279. Epub 2012 Mar 19.

Backbone

• Vector backbone: pTAL3
• Backbone manufacturer: Addgene
• Backbone size (bp): 7530
• Modifications to backbone: The pTAL5 plasmid was constructed using the Gibson method to assemble four sections into an ordered plasmid; 1 - PGAL1 was amplified from S. cerevisiae YPH500 genomic DNA. 2 - The TALEN destination protein coding sequence minus the FokI domain was amplified from pTAL3. 3 - The ADH1 terminator was amplified from pTVGI. 4 - The pTAL3 vector backbone was amplified as a linear fragment.
• Vector type: Yeast Expression
• Promoter: PGAL1
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Plasmid derived from pTAL3 from Addgene TALEN Golden Gate cloning kit.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information on Ellis TALEN Add-On Plasmids please refer to: http://www.addgene.org/TALeffector/goldengate/add-ons/#ellis