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Takashi Yamamoto Lab Plasmids


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Title Authors Publication
Efficient TALEN construction and evaluation methods for human cell and animal applications. Sakuma T, Hosoi S, Woltjen K, Suzuki KI, Kashiwagi K, Wada H, Ochiai H, Miyamoto T, Kawai N, Sasakura Y, Matsuura S, Okada Y, Kawahara A, Hayashi S, Yamamoto T Genes Cells. 2013 Feb 6. doi: 10.1111/gtc.12037.
Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379.
Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sakuma T, Nishikawa A, Kume S, Chayama K, Yamamoto T Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400.
Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes. Nakagawa Y, Sakuma T, Sakamoto T, Ohmuraya M, Nakagata N, Yamamoto T BMC Biotechnol. 2015 May 22;15(1):33.
MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems. Sakuma T, Nakade S, Sakane Y, Suzuki KT, Yamamoto T Nat Protoc. 2016 Jan;11(1):118-33. doi: 10.1038/nprot.2015.140. Epub 2015 Dec 17.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
43848pFUS_A1ALacZ + BsaI restriction sitesEfficient TALEN construction and evaluation methods for human cell and animal applications. Genes Cells. 2013 Feb 6. doi: 10.1111/gtc.12037. pCR8 Add to Cart
43849pFUS_A2ALacZ + BsaI restriction sitesEfficient TALEN construction and evaluation methods for human cell and animal applications. Genes Cells. 2013 Feb 6. doi: 10.1111/gtc.12037. pCR8 Add to Cart
43850pFUS_A2BLacZ + BsaI restriction sitesEfficient TALEN construction and evaluation methods for human cell and animal applications. Genes Cells. 2013 Feb 6. doi: 10.1111/gtc.12037. pCR8 Add to Cart
43851pFUS_A3ALacZ + BsaI restriction sitesEfficient TALEN construction and evaluation methods for human cell and animal applications. Genes Cells. 2013 Feb 6. doi: 10.1111/gtc.12037. pCR8 Add to Cart
43852pFUS_A3BLacZ + BsaI restriction sitesEfficient TALEN construction and evaluation methods for human cell and animal applications. Genes Cells. 2013 Feb 6. doi: 10.1111/gtc.12037. pCR8 Add to Cart
43853pFUS_A4ALacZ + BsaI restriction sitesEfficient TALEN construction and evaluation methods for human cell and animal applications. Genes Cells. 2013 Feb 6. doi: 10.1111/gtc.12037. pCR8 Add to Cart
43854pFUS_A4BLacZ + BsaI restriction sitesEfficient TALEN construction and evaluation methods for human cell and animal applications. Genes Cells. 2013 Feb 6. doi: 10.1111/gtc.12037. pCR8 Add to Cart
43855pcDNA-TAL-NC2TALEN-FokI nuclease backboneMammalian ExpressionEfficient TALEN construction and evaluation methods for human cell and animal applications. Genes Cells. 2013 Feb 6. doi: 10.1111/gtc.12037. pcDNA3.1 Add to Cart
43856pCAGGS-TAL-NC2TALEN-FokI nuclease backboneMammalian ExpressionEfficient TALEN construction and evaluation methods for human cell and animal applications. Genes Cells. 2013 Feb 6. doi: 10.1111/gtc.12037. pCAGGS
  • Tags / Fusion Proteins
    • FokI nuclease (C terminal on insert)
    • Flag (N terminal on insert)
  • Add to Cart
    50664p1HDrepeat 1HD (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Contains first TALE repeat harboring HD as RVD pBSK Add to Cart
    50665p1NGrepeat 1NG (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Contains first TALE repeat harboring NG as RVD pBSK Add to Cart
    50666p1NIrepeat 1NI (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Contains first TALE repeat harboring NI as RVD pBSK Add to Cart
    50667p1NNrepeat 1NN (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Contains first TALE repeat harboring NN as RVD pBSK Add to Cart
    50668p2HDrepeat 2HD (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Contains second TALE repeat harboring HD as RVD pBSK Add to Cart
    50669p2NGrepeat 2NG (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Contains second TALE repeat harboring NG as RVD pBSK Add to Cart
    50670p2NIrepeat 2NI (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Contains second TALE repeat harboring NI as RVD pBSK Add to Cart
    50671p2NNrepeat 2NN (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Contains second TALE repeat harboring NN as RVD pBSK Add to Cart
    50672p3HDrepeat 3HD (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Contains third TALE repeat harboring HD as RVD pBSK Add to Cart
    50673p3NGrepeat 3NG (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Contains third TALE repeat harboring NG as RVD pBSK Add to Cart
    50674p3NIrepeat 3NI (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Contains third TALE repeat harboring NI as RVD pBSK Add to Cart
    50675p3NNrepeat 3NN (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Contains third TALE repeat harboring NN as RVD pBSK Add to Cart
    50676p4HDrepeat 4HD (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Contains fourth TALE repeat harboring HD as RVD pBSK Add to Cart
    50677p4NGrepeat 4NG (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Contains fourth TALE repeat harboring NG as RVD pBSK Add to Cart
    50678p4NIrepeat 4NI (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Contains fourth TALE repeat harboring NI as RVD pBSK Add to Cart
    50679p4NNrepeat 4NN (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Contains fourth TALE repeat harboring NN as RVD pBSK Add to Cart
    50680pFUS2_a1aLacZ + BsaI restriction sites (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Intermediate array vector pCR8 Add to Cart
    50681pFUS2_a2aLacZ + BsaI restriction sites (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Intermediate array vector pCR8 Add to Cart
    50682pFUS2_a2bLacZ + BsaI restriction sites (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Intermediate array vector pCR8 Add to Cart
    50683pFUS2_a3aLacZ + BsaI restriction sites (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Intermediate array vector pCR8 Add to Cart
    50684pFUS2_a3bLacZ + BsaI restriction sites (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Intermediate array vector pCR8 Add to Cart
    50685pFUS2_a4aLacZ + BsaI restriction sites (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Intermediate array vector pCR8 Add to Cart
    50686pFUS2_a4bLacZ + BsaI restriction sites (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Intermediate array vector pCR8 Add to Cart
    50695pFUS2_b1LacZ + BsaI restriction sites (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Intermediate array vector pCR8 Add to Cart
    50696pFUS2_b2LacZ + BsaI restriction sites (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Intermediate array vector pCR8 Add to Cart
    50697pFUS2_b3LacZ + BsaI restriction sites (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Intermediate array vector pCR8 Add to Cart
    50698pFUS2_b4LacZ + BsaI restriction sites (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Intermediate array vector pCR8 Add to Cart
    50699ptCMV-136/63-VR-HDTALEN-FokI nuclease backbone (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Final destination vector pCMV Add to Cart
    50700ptCMV-136/63-VR-NGTALEN-FokI nuclease backbone (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Final destination vector pCMV Add to Cart
    50701ptCMV-136/63-VR-NITALEN-FokI nuclease backbone (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Final destination vector pCMV Add to Cart
    50702ptCMV-136/63-VR-NNTALEN-FokI nuclease backbone (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Final destination vector pCMV Add to Cart
    50703ptCMV-153/47-VR-HDTALEN-FokI nuclease backbone (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Final destination vector pCMV Add to Cart
    50704ptCMV-153/47-VR-NGTALEN-FokI nuclease backbone (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Final destination vector pCMV Add to Cart
    50705ptCMV-153/47-VR-NITALEN-FokI nuclease backbone (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Final destination vector pCMV Add to Cart
    50706ptCMV-153/47-VR-NNTALEN-FokI nuclease backbone (Other)TALENRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. Final destination vector pCMV Add to Cart
    58766pX330A-1x2humanized S. pyogenes Cas9 nuclease (Other)Mammalian Expression, CRISPRMultiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400. Expresses Cas9 nuclease and gRNA pUC ori vector Add to Cart
    58767pX330A-1x3humanized S. pyogenes Cas9 nuclease (Other)Mammalian Expression, CRISPRMultiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400. Expresses Cas9 nuclease and gRNA pUC ori vector Add to Cart
    58768pX330A-1x4humanized S. pyogenes Cas9 nuclease (Other)Mammalian Expression, CRISPRMultiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400. Expresses Cas9 nuclease and gRNA pUC ori vector Add to Cart
    58769pX330A-1x5humanized S. pyogenes Cas9 nuclease (Other)Mammalian Expression, CRISPRMultiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400. Expresses Cas9 nuclease and gRNA pUC ori vector Add to Cart
    58770pX330A-1x6humanized S. pyogenes Cas9 nuclease (Other)Mammalian Expression, CRISPRMultiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400. Expresses Cas9 nuclease and gRNA pUC ori vector Add to Cart
    58771pX330A-1x7humanized S. pyogenes Cas9 nuclease (Other)Mammalian Expression, CRISPRMultiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400. Expresses Cas9 nuclease and gRNA pUC ori vector Add to Cart
    58772pX330A_D10A-1x2humanized S. pyogenes Cas9 (D10A) nickase (Other)Mammalian Expression, CRISPRMultiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400. Expresses Cas9 nickase and gRNA pUC ori vector Add to Cart
    58773pX330A_D10A-1x3humanized S. pyogenes Cas9 (D10A) nickase (Other)Mammalian Expression, CRISPRMultiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400. Expresses Cas9 nickase and gRNA pUC ori vector Add to Cart
    58774pX330A_D10A-1x4humanized S. pyogenes Cas9 (D10A) nickase (Other)Mammalian Expression, CRISPRMultiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400. Expresses Cas9 nickase and gRNA pUC ori vector Add to Cart
    58775pX330A_D10A-1x5humanized S. pyogenes Cas9 (D10A) nickase (Other)Mammalian Expression, CRISPRMultiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400. Expresses Cas9 nickase and gRNA pUC ori vector Add to Cart
    58776pX330A_D10A-1x6humanized S. pyogenes Cas9 (D10A) nickase (Other)Mammalian Expression, CRISPRMultiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400. Expresses Cas9 nickase and gRNA pUC ori vector Add to Cart
    58777pX330A_D10A-1x7humanized S. pyogenes Cas9 (D10A) nickase (Other)Mammalian Expression, CRISPRMultiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400. Expresses Cas9 nickase and gRNA pUC ori vector Add to Cart
    58778pX330S-2humanized S. pyogenes Cas9 nuclease (Other)Mammalian Expression, CRISPRMultiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400. Expresses Cas9 nuclease and gRNA pUC ori vector Add to Cart
    58779pX330S-3humanized S. pyogenes Cas9 nuclease (Other)Mammalian Expression, CRISPRMultiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400. Expresses Cas9 nuclease and gRNA pUC ori vector Add to Cart
    58780pX330S-4humanized S. pyogenes Cas9 nuclease (Other)Mammalian Expression, CRISPRMultiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400. Expresses Cas9 nuclease and gRNA pUC ori vector Add to Cart
    58781pX330S-5humanized S. pyogenes Cas9 nuclease (Other)Mammalian Expression, CRISPRMultiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400. Expresses Cas9 nuclease and gRNA pUC ori vector Add to Cart
    58782pX330S-6humanized S. pyogenes Cas9 nuclease (Other)Mammalian Expression, CRISPRMultiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400. Expresses Cas9 nuclease and gRNA pUC ori vector Add to Cart
    58783pX330S-7humanized S. pyogenes Cas9 nuclease (Other)Mammalian Expression, CRISPRMultiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400. Expresses Cas9 nuclease and gRNA pUC ori vector Add to Cart
    63596pX330A_dCas9-1x2humanized S. pyogenes dCas9 (Other)Mammalian Expression, CRISPRProduction of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes. BMC Biotechnol. 2015 May 22;15(1):33. Expresses dCas9 and gRNA pUC ori vector Add to Cart
    63597pX330A_dCas9-1x3humanized S. pyogenes dCas9 (Other)Mammalian Expression, CRISPRProduction of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes. BMC Biotechnol. 2015 May 22;15(1):33. Expresses dCas9 and gRNA pUC ori vector Add to Cart
    63598pX330A_dCas9-1x4humanized S. pyogenes dCas9 (Other)Mammalian Expression, CRISPRProduction of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes. BMC Biotechnol. 2015 May 22;15(1):33. Expresses dCas9 and gRNA pUC ori vector Add to Cart
    63599pX330A_dCas9-1x5humanized S. pyogenes dCas9 (Other)Mammalian Expression, CRISPRProduction of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes. BMC Biotechnol. 2015 May 22;15(1):33. Expresses dCas9 and gRNA pUC ori vector Add to Cart
    63600pX330A_dCas9-1x6humanized S. pyogenes dCas9 (Other)Mammalian Expression, CRISPRProduction of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes. BMC Biotechnol. 2015 May 22;15(1):33. Expresses dCas9 and gRNA pUC ori vector Add to Cart
    63601pX330A_dCas9-1x7humanized S. pyogenes dCas9 (Other)Mammalian Expression, CRISPRProduction of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes. BMC Biotechnol. 2015 May 22;15(1):33. Expresses dCas9 and gRNA pUC ori vector Add to Cart
    63602pX330A_FokI-1x2FokI-dCas9 (Other)Mammalian Expression, CRISPRProduction of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes. BMC Biotechnol. 2015 May 22;15(1):33. Expresses FokI-dCas9 and gRNA pUC ori vector Add to Cart
    63603pX330A_FokI-1x3FokI-dCas9 (Other)Mammalian Expression, CRISPRProduction of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes. BMC Biotechnol. 2015 May 22;15(1):33. Expresses FokI-dCas9 and gRNA pUC ori vector Add to Cart
    63604pX330A_FokI-1x4FokI-dCas9 (Other)Mammalian Expression, CRISPRProduction of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes. BMC Biotechnol. 2015 May 22;15(1):33. Expresses FokI-dCas9 and gRNA pUC ori vector Add to Cart
    63605pX330A_FokI-1x5FokI-dCas9 (Other)Mammalian Expression, CRISPRProduction of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes. BMC Biotechnol. 2015 May 22;15(1):33. Expresses FokI-dCas9 and gRNA pUC ori vector Add to Cart
    63606pX330A_FokI-1x6FokI-dCas9 (Other)Mammalian Expression, CRISPRProduction of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes. BMC Biotechnol. 2015 May 22;15(1):33. Expresses FokI-dCas9 and gRNA pUC ori vector Add to Cart
    63607pX330A_FokI-1x7FokI-dCas9 (Other)Mammalian Expression, CRISPRProduction of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes. BMC Biotechnol. 2015 May 22;15(1):33. Expresses FokI-dCas9 and gRNA pUC ori vector Add to Cart
    63670pX330S-2-PITChhumanized S. pyogenes Cas9 (Other)Mammalian Expression, CRISPRMMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems. Nat Protoc. 2016 Jan;11(1):118-33. doi: 10.1038/nprot.2015.140. Epub 2015 Dec 17. Expresses Cas9 nuclease and the PITCh-gRNA pUC ori vector Add to Cart
    63671pX330A-FBL/PITChhumanized S. pyogenes Cas9 (Other)Mammalian Expression, CRISPRMMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems. Nat Protoc. 2016 Jan;11(1):118-33. doi: 10.1038/nprot.2015.140. Epub 2015 Dec 17. Expresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNA pUC ori vector Add to Cart
    63672pCRIS-PITChv2-FBLEGFP-2A-PuroR (Synthetic)CRISPRMMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems. Nat Protoc. 2016 Jan;11(1):118-33. doi: 10.1038/nprot.2015.140. Epub 2015 Dec 17. PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus pUC ori vector Add to Cart