Joung Lab TAL Effector Engineering Reagents
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The Joung Lab has developed three platforms for engineering TAL effector repeat arrays of any desired length: the REAL (Restriction Enzyme And Ligation) assembly method (Sander et al., Nat Biotechnol. 2011), the REAL-Fast assembly method (Reyon et al., manuscript submitted), and the FLASH (Fast Ligation-based Automatable Solid-phase High-throughput) assembly method (Reyon and Tsai et al., Nat Biotechnol 2012). All three of these platforms assemble TAL effector repeat arrays on a particular architectural framework that has yielded TALENs which function efficiently in C. elegans (Wood et al., Science 2011), zebrafish (Sander et al., Nat Biotechnol. 2011), rats (Tesson et al., Nat Biotechnol. 2011), and human somatic (Miller et al., Nat Biotechnol. 2011; Reyon and Tsai et al., Nat Biotechnol 2012) and pluripotent stem cells (Hockemeyer et al., Nat Biotechnol. 2011).
More detailed information about REAL, REAL-Fast and FLASH, including detailed and up-to-date protocols, can be found on the TALengineering website http://www.TALengineering.org Users may also wish to join the TALengineering newsgroup https://groups.google.com/group/talengineering
An upgraded version of the ZiFiT Targeter program is available to enable users to identify TALEN target sites and to simplify the construction of TALEN expression plasmids using REAL or REAL-Fast assembly. This new version of ZiFiT Targeter enables users to search their sequences of interest for potential TALEN target sites and then generates individual user-friendly graphical roadmaps for each TALEN that guide the user through the REAL or REAL-Fast assembly process. The software is publicly available without registration at: http://zifit.partners.org.
Links to additional pages describing these reagents are provided below:
Joung Lab REAL Assembly TALEN Kit
Individual REAL TALE Repeat Plasmids
TALEN Expression Vectors for REAL, REAL-Fast and FLASH
TALENs for Endogenous Zebrafish Genes
TALENs for Endogenous Human Genes (coming soon)
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