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Bernstein and Mendenhall Labs TALE-LSD1 Plasmids Available from Addgene


The TALE plasmid vectors described on this page are designed to target and edit the epigenome of putative enhancers in mammalian cells. These plasmids allow for cloning DNA fragments encoding TAL effector repeat arrays (assembled using the REAL, REAL-Fast, or FLASH methods) for expression as TALE-LSD1 fusions.

It was recently shown that TALE fusions with the histone demethylase LSD1 (Lysine specific demethylase) made using these plasmids along with the FLASH method can decrease the amount of Histone H3 lysine 4 (H3K4) di-methylation at the target locus ( Mendenhall et al., 2013 ). This epigenome editing was also shown to alter regulation of nearby gene expression.

mendenhallTALE-LSD1.png

The vectors listed below have the EF1α promoter for efficient mammalian cell expression, and encode one of three 0.5 TALE repeats (C, T, and G) and the full length human LSD1 at the carboxy-terminus of the protein.

These plasmids are described in:

Locus-specific editing of histone modifications at endogenous enhancers. Mendenhall EM, Williamson KE, Reyon D, Zou JY, Ram O, Joung JK, Bernstein BE. Nat. Biotechnol . 2013. Sep 8. doi: 10.1038/nbt.2701 . PubMed .

Individual plasmids can be ordered via the links below:

ID Plasmid Demethylase Domain C-terminal 0.5 TALE repeat Order
49042 EMM65 : Backbone contains SHD/C 0.5 Domain TALE
N-terminal: TAL C-terminus: LSD1: Isoform 2
LSD1 HD (binds C) Add to Cart
49043 EMM67 : Backbone contains SHD/G 0.5 Domain TALE
N-terminal: TAL C-terminus: LSD1: Isoform 2
LSD1 NN (binds G) Add to Cart
49044 EMM71T : Backbone contains SHD/T 0.5 Domain TALE
N-terminal: TAL C-terminus: LSD1: Isoform 2
LSD1 NG (binds T) Add to Cart