Purpose(Empty Backbone) Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40131||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 4814
Vector typeMammalian Expression
- Promoter CAG
/ Fusion Protein
- FokI nuclease (KKR) (C terminal on insert)
Growth in Bacteria
Copy numberHigh Copy
- 5′ sequencing primer TAL_F1 (ttggcgtcggcaaacagtgg)
- 3′ sequencing primer TAL_R2 (ggcgacgaggtggtcgttg) (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe TALEN destination cassette is derived from pTAL4, which is included in the Voytas Lab Golden Gate TALEN Kit. The heterodimeric FokI nuclease, including I538K, E490K, and H537R mutations as well as "Sharkey" mutations S418P and K441E, was derived from Plasmid 37199: pCMV-RosaR4 KKR mutations provided by the Gersbach lab.
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
For more information on Pelczar TALEN Add-On Plasmids please refer to: http://www.addgene.org/TALeffector/goldengate/add-ons/#pelczar
Please note that plasmid #40131 is only functional in combination with plasmid #40132.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination was a gift from Pawel Pelczar (Addgene plasmid # 40131 ; http://n2t.net/addgene:40131 ; RRID:Addgene_40131)
For your References section:Mouse genome engineering using designer nucleases. Hermann M, Cermak T, Voytas DF, Pelczar P. J Vis Exp. 2014 Apr 2;(86). doi: 10.3791/50930. 10.3791/50930 PubMed 24747757