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pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination
(Plasmid #40131)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40131 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDIRECT
  • Backbone manufacturer
    CLONTECH
  • Backbone size (bp) 4814
  • Vector type
    Mammalian Expression
  • Promoter CAG
  • Tag / Fusion Protein
    • FokI nuclease (KKR) (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The TALEN destination cassette is derived from pTAL4, which is included in the Voytas Lab Golden Gate TALEN Kit. The heterodimeric FokI nuclease, including I538K, E490K, and H537R mutations as well as "Sharkey" mutations S418P and K441E, was derived from Plasmid 37199: pCMV-RosaR4 KKR mutations provided by the Gersbach lab.
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

For more information on Pelczar TALEN Add-On Plasmids please refer to: http://www.addgene.org/TALeffector/goldengate/add-ons/#pelczar

Please note that plasmid #40131 is only functional in combination with plasmid #40132.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination was a gift from Pawel Pelczar (Addgene plasmid # 40131)
  • For your References section:

    Mouse genome engineering using designer nucleases. Hermann M, Cermak T, Voytas DF, Pelczar P. J Vis Exp. 2014 Apr 2;(86). doi: 10.3791/50930. 10.3791/50930 PubMed 24747757