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p3 FLAG_CMV14/B0,+
(Plasmid #112798)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112798 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 112798-D50 50 μg of DNA in Tris buffer $495
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DNA 112798-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    p3 FLAG_CMV14
  • Backbone manufacturer
    Sigma
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    B(0,+)/SLC6A14
  • Alt name
    Amino acid transporter ATB0,+
  • Species
    R. norvegicus (rat)
  • Mutation
    L350S
  • Entrez Gene
    Slc6a14 (a.k.a. Atb0+)
  • Tag / Fusion Protein
    • 3 x Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert sequence harbors a mutation that results in L350S relative to Genbank NP_001032633.1

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3 FLAG_CMV14/B0,+ was a gift from Katarzyna Nalęcz (Addgene plasmid # 112798 ; http://n2t.net/addgene:112798 ; RRID:Addgene_112798)

  • For your References section:

    Protein kinase C regulates amino acid transporter ATB(0,+). Samluk L, Czeredys M, Skowronek K, Nalecz KA. Biochem Biophys Res Commun. 2012 May 25;422(1):64-9. doi: 10.1016/j.bbrc.2012.04.106. Epub 2012 Apr 27. 10.1016/j.bbrc.2012.04.106 PubMed 22561015
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