Skip to main content
Addgene
Open Menu

pRL-TK CXCR4 1P
(Plasmid #11309)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11309 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 11309-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 11309-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    pRL-TK
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4045
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    1 perfect bind site for CXCR4 siRNA antisense
  • Insert Size (bp)
    46
  • Tag / Fusion Protein
    • Rr-luc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer See map
  • 3′ sequencing primer EBV-rev
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The end of the ORF and the beginning of the 3' UTR is (with the TAA
stop codon, followed by T, then the XbaI site TCTAGA):
AAATGAACAA TAA T TCTAGA GCGGCCGCT.

We digested the plasmids with XbaI, and ligated in the following:
ctaga ttccgagatatcggtaat gggcc.

This produced a modified vector, still containing an XbaI site, but
now also containing an ApaI site (GGGCCC), to allow for directional
cloning of 3' UTR inserts. The final vector contains, starting with
the stop codon TAA: TAA T Tctaga ttccgagatatcggtaat gggccC TAGA.

All UTR inserts were then constructed in the following format:
TCTAGA CTCGAGCCGG[binding site]ATCGC GGGCCC. A single perfect site
sequence is AAGTTTTCACTCCAGCTAACACCGG.

CXCR4 siRNA used is GUUUUCACUCCAGCUAACACATTCAAAAGUGAGGUCGAUUGU.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRL-TK CXCR4 1P was a gift from Phil Sharp (Addgene plasmid # 11309 ; http://n2t.net/addgene:11309 ; RRID:Addgene_11309)

  • For your References section:

    siRNAs can function as miRNAs. Doench JG, Petersen CP, Sharp PA. Genes Dev. 2003 Feb 15. 17(4):438-42. 10.1101/gad.1064703 PubMed 12600936
Related items:
Commonly requested with: