pTW322
(Plasmid
#115953)
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PurposeDestination Vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 115953 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLvl1 P1 With 3'UTR
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SpeciesSynthetic
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTW322 was a gift from Ron Weiss (Addgene plasmid # 115953 ; http://n2t.net/addgene:115953 ; RRID:Addgene_115953) -
For your References section:
Small-molecule-based regulation of RNA-delivered circuits in mammalian cells. Wagner TE, Becraft JR, Bodner K, Teague B, Zhang X, Woo A, Porter E, Alburquerque B, Dobosh B, Andries O, Sanders NN, Beal J, Densmore D, Kitada T, Weiss R. Nat Chem Biol. 2018 Nov;14(11):1043-1050. doi: 10.1038/s41589-018-0146-9. Epub 2018 Oct 16. 10.1038/s41589-018-0146-9 PubMed 30327560