pETDest
(Plasmid
#124245)
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Purpose(Empty Backbone) Allows Gateway cloning into the bacterial 6xHIS tagged protein expression vector pET26
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 124245 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28b
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Modifications to backbonepelB signal sequence was removed during the cloning
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pETDest was a gift from Attila Fehér (Addgene plasmid # 124245 ; http://n2t.net/addgene:124245 ; RRID:Addgene_124245)