pTRE-tight2-Tmem98-FLAG
(Plasmid
#124506)
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PurposeExpresses FLAG-tagged mouse TMEM98 in mammalian cells in a doxycycline-dependent manner (C-terminal tag)
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Depositing Lab
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Depositing OrganizationUniversity of Amsterdam
Located in the Netherlands -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 124506 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 124506-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 124506-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepTretight-2
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Backbone manufacturerhttps://www.addgene.org/19407/
- Backbone size w/o insert (bp) 4200
- Total vector size (bp) 4900
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Modifications to backbonean SV40-puromycin-polyA resistance cassette was inserted into the backbone to allow selection in mammalian cells
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTmem98
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SpeciesM. musculus (mouse)
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Insert Size (bp)770
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Entrez GeneTmem98 (a.k.a. 6530411B15Rik, AI463522)
- Promoter tetO-CMV
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Tag
/ Fusion Protein
- Flag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SV40 polyA
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/512426v1 for BioRxiv preprint
DNA (Catalog # 124506-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 124506-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTRE-tight2-Tmem98-FLAG was a gift from Renee van Amerongen (Addgene plasmid # 124506 ; http://n2t.net/addgene:124506 ; RRID:Addgene_124506) -
For your References section:
TMEM98 is a negative regulator of FRAT mediated Wnt/ss-catenin signalling. van der Wal T, Lambooij JP, van Amerongen R. PLoS One. 2020 Jan 21;15(1):e0227435. doi: 10.1371/journal.pone.0227435. eCollection 2020. 10.1371/journal.pone.0227435 PubMed 31961879
Map uploaded by the depositor.
