pBait
(Plasmid
#165146)
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Purpose(Empty Backbone) Bait plasmid for PROBER (empty backbone encoding replaceable ccdB cassette)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 165146 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC-like
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Backbone manufacturer6207
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Some parts of the plasmid were synthesized by GeneArt (Thermo Fisher)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBait was a gift from Paul Khavari (Addgene plasmid # 165146 ; http://n2t.net/addgene:165146 ; RRID:Addgene_165146) -
For your References section:
PROBER identifies proteins associated with programmable sequence-specific DNA in living cells. Mondal S, Ramanathan M, Miao W, Meyers RM, Rao D, Lopez-Pajares V, Siprashvili Z, Reynolds DL, Porter DF, Ferguson I, Neela P, Zhao Y, Meservey LM, Guo M, Yang YY, Li L, Wang Y, Khavari PA. Nat Methods. 2022 Aug;19(8):959-968. doi: 10.1038/s41592-022-01552-w. Epub 2022 Aug 4. 10.1038/s41592-022-01552-w PubMed 35927480