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pGilesSHdI_redgreen_2395A_His6_triple red_pLeft1806_His6_double green
(Plasmid #170048)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170048 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 170048-D50 50 μg of DNA in Tris buffer $495
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DNA 170048-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pGIlesSHdI
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 200 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    mScarlet
  • Insert Size (bp)
    692

Gene/Insert 2

  • Gene/Insert name
    COmScarlet
  • Insert Size (bp)
    696

Gene/Insert 3

  • Gene/Insert name
    mRuby3
  • Insert Size (bp)
    711

Gene/Insert 4

  • Gene/Insert name
    mWasabi dN
  • Insert Size (bp)
    717

Gene/Insert 5

  • Gene/Insert name
    mWasabi CO

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGilesSHdI_redgreen_2395A_His6_triple red_pLeft1806_His6_double green was a gift from Clifton Barry III (Addgene plasmid # 170048 ; http://n2t.net/addgene:170048 ; RRID:Addgene_170048)

  • For your References section:

    Development and Optimization of Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs. Kolbe K, Bell AC, Prosser GA, Assmann M, Yang HJ, Forbes HE, Gallucci S, Mayer-Barber KD, Boshoff HI, Barry Iii CE. Front Microbiol. 2020 Dec 9;11:591866. doi: 10.3389/fmicb.2020.591866. eCollection 2020. 10.3389/fmicb.2020.591866 PubMed 33362741
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