pEF6 VPS51-V5-6His
(Plasmid
#175502)
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PurposeV5 tagged VPS51 is expressed in mammalian cells
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Depositing Lab
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Depositing OrganizationNational Institute of Child Health and Human Development (NICHD)
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 175502 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 175502-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 175502-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEF6-V5-His
- Backbone size w/o insert (bp) 5840
- Total vector size (bp) 8186
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVPS51
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Alt nameANG2
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SpeciesH. sapiens (human)
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Insert Size (bp)2346
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MutationA661V- please see depositor comments
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Entrez GeneVPS51 (a.k.a. ANG2, ANG3, C11orf2, C11orf3, FFR, PCH13)
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Tag
/ Fusion Protein
- V5 (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Depositor confirms A661V mutation does not affect plasmid function.
DNA (Catalog # 175502-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 175502-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF6 VPS51-V5-6His was a gift from Juan Bonifacino (Addgene plasmid # 175502 ; http://n2t.net/addgene:175502 ; RRID:Addgene_175502) -
For your References section:
Ang2/fat-free is a conserved subunit of the Golgi-associated retrograde protein complex. Perez-Victoria FJ, Schindler C, Magadan JG, Mardones GA, Delevoye C, Romao M, Raposo G, Bonifacino JS. Mol Biol Cell. 2010 Oct 1;21(19):3386-95. doi: 10.1091/mbc.E10-05-0392. Epub 2010 Aug 4. 10.1091/mbc.E10-05-0392 PubMed 20685960
