pGC180
(Plasmid
#19616)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 19616 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepGC31
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Vector typeWorm Expression ; FLP/FRT, Destination vector
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Growth instructionsDB3.1 cells
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGateway(attR1-attR2)::FLP::let-858(3’)-(Cbr)unc-119(+)
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SpeciesC. elegans (nematode), S. cerevisiae (budding yeast)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGC180 was a gift from Jane Hubbard (Addgene plasmid # 19616 ; http://n2t.net/addgene:19616 ; RRID:Addgene_19616) -
For your References section:
A "FLP-Out" system for controlled gene expression in Caenorhabditis elegans. Voutev R, Hubbard EJ. Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274. 10.1534/genetics.108.090274 PubMed 18723890
